| Description | IDG-SW3 represents a heterogeneous population that develops from early osteoblasts to late osteocytes. |
| Tissue/Organ/Organ System | Skeletal |
| Immortalization Method | Isolated from long bones of transgenic mice (in which the Dmp1 promoter drives the expression of GFP). |
| Cell Morphology | Polygonal |
| Growth Properties | Adherent |
| Seeding Density | 25,000 - 30,000 cells/cm2 |
| Population Doubling Time | 40 - 50 hours |
| Expression Profile | ALP (osteoblast), E11/gp38, Dmp1 and Phex (osteocyte), SOST/sclerostin and FGF23 (late osteocyte) |
| Quality Control | 1) The presence and absence of SV40 big T antigen are confirmed by Western blot under permitted and non-permitted conditions. 2) Fluorescence imaging is used to measure the expression of GFP driven by the Dmp1 promoter under osteogenic conditions. 3) Von Kossa staining showed focal nodule mineralization, Alizarin Red S staining detected calcium deposition in differentiated cells. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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