| Description | Immortalized mouse preadipocytes were isolated from the subcutaneous adipose tissue of C57BL/6 mice, and were immortalized by infection with a retrovirus carrying the hTERT gene. |
| Tissue/Organ/Organ System | Adipose |
| Immortalization Method | Serial passaging and infection with retroviruses carrying hTERT gene |
| Cell Morphology | Fibroblast-like |
| Growth Properties | Adherent |
| Seeding Density | 20,000 - 50,000 cells/cm2 |
| Population Doubling Time | 16 - 26 hours |
| Quality Control | 1) Oil Red O staining at day 7 after inducing adipocyte differentiation to visualize lipid droplets; 2) Harvest RNA after induction of adipogenesis and perform RT-PCR and Northern Blot to detect transcript level of adipocyte markers SCD1, aFABP, ATGL, FAS, LDL, and GPDH B. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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