| Description | Immortalized mouse retinal pigment epithelial cells-HPV E6/E7, derived from healthy C57B1/6 mouse RPE cells, is a cell line that retains many in vivo phenotypic characteristics. |
| Tissue/Organ/Organ System | Eye |
| Immortalization Method | Serial passaging and transduction with retrovirus carrying human papiloma virus 16 E6/E7 |
| Cell Morphology | Cobble-stone |
| Growth Properties | Adherent |
| Seeding Density | 5,000 - 10,000 cells/cm2 |
| Population Doubling Time | ~50 hours |
| Expression Profile | RPE65, CRALBP, ZO1, Cytokeratin -8 and -18 |
| Quality Control | 1. Western Blot was used to verify the HPV protein expression in the immortalized cell line. 2. Western blot and real-time RT-PCR was used to confirm the presence of in vivo RPE markers such as RPE65 and CRALBP. 3. Immunofluorescence staining was used to confirm expression of epithelial cell markers ZO1, cytokeratin 8 and cytokeratin 18. 4. Absence of neural cell marker, SV2, PKCα, rhodopsin |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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