| Description | MSIE is a conditionally immortalized mouse small intestinal epithelial cell line, which was isolated from mice carrying heat-labile mutations |
| Tissue/Organ/Organ System | Digestive |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1) Immunohistochemical staining was used to detect the presence of SV40 large T antigen and keratin 18; 2) Biochemical assays were used to measure brush border-associated enzymes alkaline phosphatase, dipeptidyl peptidase IV and sucrase. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 15,000 - 25,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Other |
| Expression Profile | CK18 |
| Immortalization Method | Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58) |
| Population Doubling Time | 35 - 45 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
