| Description | Immortalized human gastric cells (KMU-CS12) are also valuable in cancer research, especially in the etiology of gastric cancer and research on new treatment strategies. |
| Tissue/Organ/Organ System | Spleen |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1. Immunofluorescence microscopy analysis confirmed SRDC maturation- through expression of DC-LAMP and MHC-II expression. 2. ELISA assays to determine interleukin and gamma interferon production. 3. To confirm dendritic cell transformation, SV40 large T antigen expression was compared between SRDCs and Cos-7-positive control cells and BHK-21-negative control cells. 4. PCR analysis confirmed that the SV40 oncogene was not integrated into SRDC cellular DNA. 5. Antigen uptake ability tested via incubation with antitoxoplasma serum. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Seeding Density | 30,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Dendritic-like |
| Expression Profile | HuD, PGP9.5, tau, MHC-I, MHC-II, CD80, CD40, CD11c, CD59, CD205 |
| Immortalization Method | Serial passaging and liposome-mediated transfection with pSV3neo plasmid carrying the SV40 T oncogene |
| Population Doubling Time | 36 - 46 hours |
| Price | Inquiry |
