Cat. No.: ICELL-0427

Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP)

Description The lentiviral vector carrying green fluorescent protein was used to genetically modify the subcutaneous fat pluripotent mesenchymal cells of immortalized mice.
Tissue/Organ/Organ System Adipose
Immortalization Method Spontaneous immortalization
Cell Morphology Fibroblast-like
Growth Properties Adherent
Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Quality Control 1) Differentiated cells were specifically stained with Alizarin Red, Fat Red and Alcian Blue and tissue-specific type II collagen antibodies to confirm the pluripotency of the cells' osteogenic, fat, and chondrogenic phenotypes.
2) Compared with normal mouse karyotype, G-band analysis of macro chromosome changes over time.
3) Antibiotic culture confirmed GFP transduction
Size 1*106 cells/1.0 ml
Format Frozen
Storage Liquid Nitrogen
Shipping Info Dry Ice
Categories Immortalized Cells
Applications For Research Use Only
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