| Description | This cell line stably expresses stem cell markers Sca-1, c-Kit/CD117, nestin, nuclear backbone protein, CD44 and CD106, but is negative for embryonic stem cell markers Nanog, Oct4 and Sox2. |
| Tissue/Organ/Organ System | Adipose |
| Immortalization Method | Spontaneous immortalization |
| Cell Morphology | Fibroblast-like |
| Growth Properties | Adherent |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Quality Control | 1) Perform RT-PCR and cell fluorescence on cDNA from cells to confirm the expression of stem cell markers Sca-1, c-kit, pancreatic islet 1, nestin, and nuclear stem protein. 2) Differentiated cells were specifically stained with Alizarin Red, Fat Red and Alcian Blue and tissue-specific collagen II antibodies to confirm the pluripotency of the cells' osteogenic, adipogenic, and cartilage phenotypes. 3) Compared with the normal mouse karyotype, the G-band analysis of large chromosomes changes over time. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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