| Description | PAMs were isolated from ten 10-week-old clinically healthy Yorkshire pigs raised at a local pig farm. Primary porcine alveolar macrophages (PAM) are useful for studying viral infections and immune response in pigs. Immortalized PAM cells possessed the characteristics of primary PAMs including strong expression of swine leukocyte antigen (SLA) class II genes and the inability to grow anchorage-independently. iPAM303 (73.3%+-5.4; SLA DQA*0106/0201; DQB1*0202/0701; DRB1*0402/0602). |
| Donor Age | 10 weeks old |
| Tissue/Organ/Organ System | Lung |
| Immortalization Method | Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit |
| Cell Morphology | Fibroblast-like |
| Growth Properties | Adherent |
| Expression Profile | Puromycin resistance |
| Quality Control | 1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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