| Description | Activated primary rat portal fibroblasts classified by the Ntpdase2/Cd39l1 marker were immortalized with SV40 large T antigen to generate immortalized rat portal fibroblasts. |
| Tissue/Organ/Organ System | Liver |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | 1.) Gene expression analysis to confirm the expression of common liver myofibroblast-associated genes. 2.) Immunoblot and immunofluoresence microscopy to detect myofibroblast-specific cell markers. 3.) Transfection assay to confirm DNA transfectability of cells. 4.) Co-culture assay to demonstrate the inhibition of Mz-ChA-1 cholangiocarcinoma proliferation. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | 15,000 - 20,000 cells/cm2 |
| Applications | For Research Use Only |
| Cell Morphology | Spindle shaped |
| Donor Age | Adult |
| Expression Profile | Alpha smooth muscle actin, type I collagen alpha-1, tissue inhibitor of metalloproteinases-1, PF-specific markers elastin, type XV collagen alpha-1 and Ntpdase2/Cd39l1, ecto-5’-nucleotidase/Cd73 |
| Immortalization Method | Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector |
| Population Doubling Time | 17 - 27 hours |
| Price | Inquiry |
