| Description | Immortalized rat retinal capillary endothelial cells were immortalized with SV40 antigen. |
| Tissue/Organ/Organ System | Eye |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Shipping Info | Dry Ice |
| Quality Control | Real Time PCR was used to quantify SV40 gene expression in immortalized cell line. |
| Storage Conditions | Liquid Nitrogen |
| Growth Properties | Adherent |
| Categories | Immortalized Cells |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Applications | For Research Use Only |
| Cell Morphology | Polygonal |
| Immortalization Method | Serial passaging and infection with Lentivirus carrying SV40 antigen gene |
| Population Doubling Time | 24-28 hours |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Price | Inquiry |
