| Description | Müller cells are glial cells in the vertebrate retina, responsible for the homeostasis and metabolic support of retinal neurons. |
| Tissue/Organ/Organ System | Eye |
| Immortalization Method | Serial passaging and Calcium Phosphate mediated transfection with simian virus 40 (SV40) DNA |
| Cell Morphology | Flattened |
| Growth Properties | Adherent |
| Seeding Density | 10,000 - 30,000 cells/cm2 |
| Population Doubling Time | 12 - 22 hours |
| Expression Profile | GFAP, CRALBP |
| Quality Control | 1) Immunocytochemistry and immunoblotting methods to detect the presence of glial fibrillary acidic protein (GFAP) and cellular retinal binding protein (CRALBP); 2) Immunocytochemistry is used to confirm the nuclear expression of SV40-LTA in immortalized cells. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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