| Description | Cell lines play an important role in regulating oogenesis, spermatogenesis and steroid hormone production. |
| Tissue/Organ/Organ System | Reproductive |
| Immortalization Method | Serial passaging and calcium phosphate mediated cotransfection by SV40 DNA and Ha-ras oncogene |
| Cell Morphology | Other |
| Growth Properties | Adherent |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Quality Control | 1) Phenotypic response to hCG stimulation was characterized by shape changes (cells rounding up as a result of intracellular cAMP accumulation); 2) LH/CG receptor expression was measured by radiolabelled 125I-hCG binding assay- the cells express 19,000 FSH receptors/cell with a Kd of 180 pM, similar to the native receptor; 3) Production of progesterone and 20α-OH-P was determined by radioimmunoassay (RIA); 4) Production of cAMP was measured by a protein binding method |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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