| Description | This cell line is a useful model for studying the pathology and treatment of SLC4A11-related congenital hereditary endothelial dystrophy and Fuchs endothelial corneal dystrophy. |
| Tissue/Organ/Organ System | Eye |
| Immortalization Method | Intercrossed the Slc4a11þ/þ and Slc4a11/C57BL/6 mice with H-2Kb-tsA58 Immortomouse, which carries a temperature-sensitive mutant form of the simian virus-40 large T antigen |
| Cell Morphology | Fibroblast-like |
| Growth Properties | Adherent |
| Population Doubling Time | 18.72 hours |
| Quality Control | 1) Western blot; 2) Intracellular pH (pHi) measurement; 3) RT-PCR; 4) Real-time quantitative PCR |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Immortalized Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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