| Description | The knockout cell line is HEK293/HeLa cell line. |
| Species | Human |
| Donor Disease | Carcinoma |
| Donor Gender | Female |
| Tissue/Organ/Organ System | Cervix/Kidney |
| Parental Cell Line | HEK293/HeLa |
| Growth Properties | Adherent |
| Culture Conditions | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
| Subculture Guidelines | All seeding densities should be based on cell counts obtained by established methods. The recommended seeding density is 2*104 cells/cm2. If necessary, a partial replacement of the medium 24 hours before subculture may help promote growth. When the cells achieve 80-90% confluence, they should be passaged. |
| Viability | ~80% |
| Purity | Immunogen affinity purified |
| Mycoplasma Free | Yes |
| Quality Control | All cells test negative for bacteria, yeast, and fungi. |
| Recommended Culture Medium And Supplement | Growth Medium: IMDM, 10% FCS. |
| Freezing Medium | Freeze Medium: IMDM, 20% FCS, 10% DMSO |
| Size | 1 vial |
| Storage | Store in liquid nitrogen. |
| Storage Buffer | Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether |
| Shipping Info | Dry Ice |
| Cell Revival | Revival: Rapidly thaw cells in a 37°C water bath. Transfer the contents to the tube containing the preheating medium. Centrifuge the cells and seeds into the pre-warmed medium in a 10 ml 10 cm Petri dish. |
| Biosafety Level | 1 |
| Product Type | Knockout Stable Cell Line |
| Knockout Validation | Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB) |
| Application | For research use only |
| Price | Inquiry |
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