| Description | Mouse SPP1 knockout 4T1 cell line is edited by our special CRISPR/Cas9 technology. 4T1 is a breast cancer cell line derived from mammary tissue of the mouse BALB/c strain. 4T1 cells are epithelial cells that are resistant to 6-thioguanine. 4T1 resembles Mouse metastatic triple-negative breast cancer (TNBC), lacking the expression of estrogen receptor (ER), progesterone receptor (PR), and epidermal growth factor receptor 2 (HER2). |
| Size | 1 vial (2*106 cells/vial) |
| Parental Cell Line | 4T1 |
| Biosafety Level | 1 |
| Format | Frozen |
| Culture Conditions | 4T1 Cells are cultured with 95% air and 5% of carbon dioxide (CO2) at 37℃. |
| Culture Medium | RPMI-1640 Medium + 10% FBS |
| Subculture Conditions | 1:6 to 1:8 using 0.25% trypsin or trypsin/EDTA. |
| Derived From | Breast tissue |
| Freezing Medium | Complete growth medium 95% with DMSO 5% is used as a freezing medium |
| Subculture Guidelines | 4T1 cells should not be allowed to reach 100% confluency. Subculture at 80% confluence is recommended. To detach cells from the surface, rinse with 0.25% trypsin-0.53 mM EDTA solution at room temperature. |
| Knockout Validation | Sanger Sequencing, Western Blot (WB) |
| Viability | >95% viability before freezing. |
| Adherent /Suspension | Adherent |
| Disease | Breast Cancer |
| Purity | Immunogen affinity purified |
| Storage | Vapor phase of liquid nitrogen |
| Sterility | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
| Shipping Info | Dry Ice |
| Species | Mouse |
| Tested Applications | Suitable for: WB |
| Tissue | Breast; Mammary gland |
| Recommended Control | Mouse wild-type 4T1 cell line |
| Doubling Time | 14 hours |
| Morphology | Epithelial |
| Cytogenic Data | Knockout Stable Cell Line |
| Applications | For research use only |
| Product Type | Knockout Stable Cell Line |
| Price | Inquiry |
