| Description | The PARP-1-/-Knockout immortalized mouse heart endothelium (HYKO6) cell line is derived from primary mouse heart endothelial cells, which have been stably transformed into PARP-1-deficient mice by SV40. |
| Species | Mouse |
| Tissue/Organ/Organ System | Heart |
| Pharmaceutical Target | Other |
| Cell Morphology | Cobble-stone |
| Growth Properties | Adherent |
| Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Expression Profile | CD105, CD31, ICAM-2, VCAM-1, vWF |
| Quality Control | 1) Flow cytometry to detect the expression of endothelial cell markers such as CD105, CD31, ICAM-2, VCAM-1 and MHC class I antigen; 2) Immunofluorescence staining to evaluate vWF expression; 3) Western blot to detect immortalized cell lines Expression of SV40 T antigen. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Drug Discovery Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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