| Description | These dendritic cell lines provide powerful tools for vaccine science and immunotherapy, especially in the development of strategies against the CD8α subset of target antigens. |
| Species | Mouse |
| Tissue/Organ/Organ System | Spleen |
| Pharmaceutical Target | Catalytic Receptors |
| Cell Morphology | Small Aggregates |
| Growth Properties | Adherent |
| Seeding Density | 20,000 – 60,000 cells/cm2. Recommended split ratio: no greater than 1:3. |
| Expression Profile | CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4 |
| Quality Control | 1) Evaluate direct antigen presentation and cross antigen presentation through MHC-I (SIINFEKL/OT-I) and MHC-II (OVA323-339/OT-II) restriction systems; 3) Proteomic profile and surface evaluation through RT-PCR Labeling; Reverse transcription polymerase chain reaction; 3) Analyze IL-12 cytokine secretion using ELISA; 4) Evaluate the response to PAMP stimulation by functional assay. |
| Size | 1*106 cells/1.0 ml |
| Format | Frozen |
| Storage | Liquid Nitrogen |
| Preservation Protocol | 1. Freeze Medium: Complete growth medium with 50% decomplemented fetal calf serum (PAN biotech; P40-37500) and 10% DMSO. 2. Storage Temperature: Liquid Nitrogen vapour phase. |
| Shipping Info | Dry Ice |
| Categories | Drug Discovery Cells |
| Applications | For Research Use Only |
| Price | Inquiry |
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