Cat. No.: SCELL-00019

TP53 (-/-); PIK3CA (H1047R/+) MCF10A Cell Line

Description The Cell line is Heterozygous knockin of PIK3CA kinase domain activating mutation in TP53 (-/-) parental cell line.
Species Human
Growth Properties The cells are cultured as a monolayer in a humid environment at 37°C and 5% CO2. Cells should be passaged every 3-5 days.
Quality Control All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Recommended Culture Medium And Supplement Growth Medium: DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 5% horse serum, 10 µg/mL RI, 0.5 µg/mL hydrocortisone, 0.1 µg/mL cholera toxin.; Freeze Medium: Complete growth media 45%; DMSO 5%; Reviv
Size 1 vial
Storage Liquid Nitrogen
Shipping Info Dry Ice
Biosafety Level 1
Stock Knockin Stable Cell Line
Application For research use only
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